Journal: Toxics

Article Title: PCB Exposure in Adult Male Mice Reduces Proliferating Cells in the Prostate but Minimally Alters Voiding

doi: 10.3390/toxics14030265

Figure Lengend Snippet: Adult PCB exposure does not alter smooth muscle actin thickness in the prostate. Adult male mice were exposed to a daily dosage of the MARBLES PCB mixture for 2 months and tissues were collected for histology. Representative images of ( A ) anterior prostate, ( B ) ventral prostate, and ( C ) dorsolateral prostate stained with antibodies targeting alpha smooth muscle actin 2 (ACTA2, green) and E-cadherin (red, all epithelium) and counterstained with DAPI to label all nuclei (blue). Quantification of smooth muscle thickness surrounding ducts in the ( D ) anterior prostate, ( E ) ventral prostate, and ( F ) dorsolateral prostate. Results are mean ± SEM, n = 4–5 per treatment group. No significant differences p ≤ 0.05, as determined by a one-way ANOVA test.

Article Snippet: Smooth Muscle Actin (Acta2) , Goat , Fisher Scientific , PA518292 RRID: AB_10980764 , 1:250 , .

Techniques: Staining